1sdm
From Proteopedia
(New page: 200px<br /><applet load="1sdm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sdm, resolution 2.30Å" /> '''Crystal structure of...) |
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| - | [[Image:1sdm.jpg|left|200px]]<br /><applet load="1sdm" size=" | + | [[Image:1sdm.jpg|left|200px]]<br /><applet load="1sdm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sdm, resolution 2.30Å" /> | caption="1sdm, resolution 2.30Å" /> | ||
'''Crystal structure of kinesin-like calmodulin binding protein'''<br /> | '''Crystal structure of kinesin-like calmodulin binding protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Kinesins orchestrate cell division by controlling placement of | + | Kinesins orchestrate cell division by controlling placement of chromosomes. Kinesins must be precisely regulated or else cell division fails. Calcium, a universal second messenger in eukaryotes, and calmodulin regulate some kinesins by causing the motor to dissociate from its biological track, the microtubule. Our focus was the mechanism of calcium regulation of kinesin at atomic resolution. Here we report the crystal structure of kinesin-like calmodulin-binding protein (KCBP) from potato, which was resolved to 2.3 A. The structure reveals three subdomains of the regulatory machinery located at the C terminus extension of the kinesin motor. Calmodulin that is activated by Ca2+ ions binds to an alpha-helix positioned on the microtubule-binding face of kinesin. A negatively charged segment following this helix competes with microtubules. A mimic of the conventional kinesin neck, connecting the calmodulin-binding helix to the KCBP motor core, links the regulatory machine to the kinesin catalytic cycle. Together with biochemical data, the crystal structure suggests that Ca(2+)-calmodulin inhibits the binding of KCBP to microtubules by blocking the microtubule-binding sites on KCBP. |
==About this Structure== | ==About this Structure== | ||
| - | 1SDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1SDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_tuberosum Solanum tuberosum] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SDM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Solanum tuberosum]] | [[Category: Solanum tuberosum]] | ||
| - | [[Category: Fletterick, R | + | [[Category: Fletterick, R J.]] |
| - | [[Category: Reddy, A | + | [[Category: Reddy, A S.]] |
| - | [[Category: Reddy, V | + | [[Category: Reddy, V S.]] |
| - | [[Category: Sablin, E | + | [[Category: Sablin, E P.]] |
| - | [[Category: Vinogradova, M | + | [[Category: Vinogradova, M V.]] |
[[Category: ADP]] | [[Category: ADP]] | ||
[[Category: MG]] | [[Category: MG]] | ||
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[[Category: minus-end directed]] | [[Category: minus-end directed]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:26 2008'' |
Revision as of 13:00, 21 February 2008
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Crystal structure of kinesin-like calmodulin binding protein
Overview
Kinesins orchestrate cell division by controlling placement of chromosomes. Kinesins must be precisely regulated or else cell division fails. Calcium, a universal second messenger in eukaryotes, and calmodulin regulate some kinesins by causing the motor to dissociate from its biological track, the microtubule. Our focus was the mechanism of calcium regulation of kinesin at atomic resolution. Here we report the crystal structure of kinesin-like calmodulin-binding protein (KCBP) from potato, which was resolved to 2.3 A. The structure reveals three subdomains of the regulatory machinery located at the C terminus extension of the kinesin motor. Calmodulin that is activated by Ca2+ ions binds to an alpha-helix positioned on the microtubule-binding face of kinesin. A negatively charged segment following this helix competes with microtubules. A mimic of the conventional kinesin neck, connecting the calmodulin-binding helix to the KCBP motor core, links the regulatory machine to the kinesin catalytic cycle. Together with biochemical data, the crystal structure suggests that Ca(2+)-calmodulin inhibits the binding of KCBP to microtubules by blocking the microtubule-binding sites on KCBP.
About this Structure
1SDM is a Single protein structure of sequence from Solanum tuberosum with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of kinesin regulated by Ca(2+)-calmodulin., Vinogradova MV, Reddy VS, Reddy AS, Sablin EP, Fletterick RJ, J Biol Chem. 2004 May 28;279(22):23504-9. Epub 2004 Feb 26. PMID:14988396
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