1fv7
From Proteopedia
(New page: 200px<br /><applet load="1fv7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fv7" /> '''A TWO B-Z JUNCTION CONTAINING DNA RESOLVES I...) |
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| - | [[Image:1fv7.gif|left|200px]]<br /><applet load="1fv7" size=" | + | [[Image:1fv7.gif|left|200px]]<br /><applet load="1fv7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1fv7" /> | caption="1fv7" /> | ||
'''A TWO B-Z JUNCTION CONTAINING DNA RESOLVES INTO AN ALL RIGHT HANDED DOUBLE HELIX'''<br /> | '''A TWO B-Z JUNCTION CONTAINING DNA RESOLVES INTO AN ALL RIGHT HANDED DOUBLE HELIX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Natural and artificial oligonucleotides are capable of assuming many | + | Natural and artificial oligonucleotides are capable of assuming many different conformations and functions. Here we present results of an NMR restrained molecular modelling study on the conformational preferences of the modified decanucleotide d((m)C1G2(m)C3G4C5(L)G6(L)(m)C7G8(m)C9G10) .d((m)C11G12(m)C13G14C15(L)G (L)16(m)C17-G18(m)C19G20 ) which contains L deoxynucleotides in its centre. This chimeric DNA was expected to form a right-left-right-handed B-type double-helix (BB*B) at low salt concentration. Actually, it matured into a fully right-handed double helix with its central C(L)pG(L) core forming a right-handed Z-DNA helix embedded in a B-DNA matrix (BZ*B). The interplay between base-base and base-sugar stackings within the core and its immediately adjacent residues was found to be critical in ensuring the stabilisation of the right-handed helix. The structure could serve as a model for the design of antisense oligonucleotides resistant to nucleases and capable of hybridising to natural DNAs and RNAs. |
==About this Structure== | ==About this Structure== | ||
| - | 1FV7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CH3 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1FV7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CH3:'>CH3</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FV7 OCA]. |
==Reference== | ==Reference== | ||
A two B-Z junction containing DNA resolves into an all right-handed double-helix., Mauffret O, El Amri C, Santamaria F, Tevanian G, Rayner B, Fermandjian S, Nucleic Acids Res. 2000 Nov 15;28(22):4403-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11071926 11071926] | A two B-Z junction containing DNA resolves into an all right-handed double-helix., Mauffret O, El Amri C, Santamaria F, Tevanian G, Rayner B, Fermandjian S, Nucleic Acids Res. 2000 Nov 15;28(22):4403-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11071926 11071926] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Amri, C | + | [[Category: Amri, C El.]] |
[[Category: Fermandjian, S.]] | [[Category: Fermandjian, S.]] | ||
[[Category: Mauffret, O.]] | [[Category: Mauffret, O.]] | ||
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[[Category: l enantiomery]] | [[Category: l enantiomery]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:42:58 2008'' |
Revision as of 10:43, 21 February 2008
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A TWO B-Z JUNCTION CONTAINING DNA RESOLVES INTO AN ALL RIGHT HANDED DOUBLE HELIX
Overview
Natural and artificial oligonucleotides are capable of assuming many different conformations and functions. Here we present results of an NMR restrained molecular modelling study on the conformational preferences of the modified decanucleotide d((m)C1G2(m)C3G4C5(L)G6(L)(m)C7G8(m)C9G10) .d((m)C11G12(m)C13G14C15(L)G (L)16(m)C17-G18(m)C19G20 ) which contains L deoxynucleotides in its centre. This chimeric DNA was expected to form a right-left-right-handed B-type double-helix (BB*B) at low salt concentration. Actually, it matured into a fully right-handed double helix with its central C(L)pG(L) core forming a right-handed Z-DNA helix embedded in a B-DNA matrix (BZ*B). The interplay between base-base and base-sugar stackings within the core and its immediately adjacent residues was found to be critical in ensuring the stabilisation of the right-handed helix. The structure could serve as a model for the design of antisense oligonucleotides resistant to nucleases and capable of hybridising to natural DNAs and RNAs.
About this Structure
1FV7 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
A two B-Z junction containing DNA resolves into an all right-handed double-helix., Mauffret O, El Amri C, Santamaria F, Tevanian G, Rayner B, Fermandjian S, Nucleic Acids Res. 2000 Nov 15;28(22):4403-9. PMID:11071926
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