1jy5
From Proteopedia
(New page: 200px<br /><applet load="1jy5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jy5, resolution 2.05Å" /> '''RNase-related protei...) |
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| - | [[Image:1jy5.gif|left|200px]]<br /><applet load="1jy5" size=" | + | [[Image:1jy5.gif|left|200px]]<br /><applet load="1jy5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1jy5, resolution 2.05Å" /> | caption="1jy5, resolution 2.05Å" /> | ||
'''RNase-related protein from Calystegia sepium'''<br /> | '''RNase-related protein from Calystegia sepium'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a catalytically inactive RNase-related protein from | + | The structure of a catalytically inactive RNase-related protein from Calystegia sepium (CalsepRRP) has been resolved by protein crystallography at a resolution of 2.05 A and an R factor of 20.74%. Although the protein is completely devoid of ribonuclease activity, it adopts the typical alpha + beta structure of non-base-specific RNases. Analysis of the structure revealed that two amino-acid substitutions in the 'active' P1 site, in combination with the less hydrophobic/aromatic character of the B1 base-recognition site and a completely disrupted B2 base-recognition site, might account for this complete lack of activity. |
==About this Structure== | ==About this Structure== | ||
| - | 1JY5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Calystegia_sepium Calystegia sepium]. Full crystallographic information is available from [http:// | + | 1JY5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Calystegia_sepium Calystegia sepium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JY5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Barre, A.]] | [[Category: Barre, A.]] | ||
| - | [[Category: Damme, E | + | [[Category: Damme, E J.M Van.]] |
| - | [[Category: Peumans, W | + | [[Category: Peumans, W J.]] |
[[Category: Rabijns, A.]] | [[Category: Rabijns, A.]] | ||
| - | [[Category: Ranter, C | + | [[Category: Ranter, C J.De.]] |
[[Category: Rouge, P.]] | [[Category: Rouge, P.]] | ||
[[Category: Verboven, C.]] | [[Category: Verboven, C.]] | ||
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[[Category: rnase]] | [[Category: rnase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:27:59 2008'' |
Revision as of 11:28, 21 February 2008
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RNase-related protein from Calystegia sepium
Overview
The structure of a catalytically inactive RNase-related protein from Calystegia sepium (CalsepRRP) has been resolved by protein crystallography at a resolution of 2.05 A and an R factor of 20.74%. Although the protein is completely devoid of ribonuclease activity, it adopts the typical alpha + beta structure of non-base-specific RNases. Analysis of the structure revealed that two amino-acid substitutions in the 'active' P1 site, in combination with the less hydrophobic/aromatic character of the B1 base-recognition site and a completely disrupted B2 base-recognition site, might account for this complete lack of activity.
About this Structure
1JY5 is a Single protein structure of sequence from Calystegia sepium. Full crystallographic information is available from OCA.
Reference
Structure of an RNase-related protein from Calystegia sepium., Rabijns A, Verboven C, Rouge P, Barre A, Van Damme EJ, Peumans WJ, De Ranter CJ, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):627-33. Epub 2002, Mar 22. PMID:11914487
Page seeded by OCA on Thu Feb 21 13:27:59 2008
