1br3
From Proteopedia
(New page: 200px<br /><applet load="1br3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1br3, resolution 3.00Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1br3.gif|left|200px]]<br /><applet load="1br3" size=" | + | [[Image:1br3.gif|left|200px]]<br /><applet load="1br3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1br3, resolution 3.00Å" /> | caption="1br3, resolution 3.00Å" /> | ||
'''CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME'''<br /> | '''CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of a large nucleic acid complex formed by the 10-23 DNA | + | The structure of a large nucleic acid complex formed by the 10-23 DNA enzyme bound to an RNA substrate was determined by X-ray diffraction at 3.0 A resolution. The 82-nucleotide complex contains two strands of DNA and two strands of RNA that form five double-helical domains. The spatial arrangement of these helices is maintained by two four-way junctions that exhibit extensive base-stacking interactions and sharp turns of the phosphodiester backbone stabilized by metal ions coordinated to nucleotides at these junctions. Although it is unlikely that the structure corresponds to the catalytically active conformation of the enzyme, it represents a novel nucleic acid fold with implications for the Holliday junction structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1BR3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1BR3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BR3 OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme., Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF, Nat Struct Biol. 1999 Feb;6(2):151-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10048927 10048927] | Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme., Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF, Nat Struct Biol. 1999 Feb;6(2):151-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10048927 10048927] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Joyce, G | + | [[Category: Joyce, G F.]] |
[[Category: Nowakowski, J.]] | [[Category: Nowakowski, J.]] | ||
| - | [[Category: Prasad, G | + | [[Category: Prasad, G S.]] |
| - | [[Category: Shim, P | + | [[Category: Shim, P J.]] |
| - | [[Category: Stout, C | + | [[Category: Stout, C D.]] |
[[Category: dna enzyme]] | [[Category: dna enzyme]] | ||
[[Category: holliday junction]] | [[Category: holliday junction]] | ||
[[Category: ribozyme]] | [[Category: ribozyme]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:58:12 2008'' |
Revision as of 09:58, 21 February 2008
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CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME
Overview
The structure of a large nucleic acid complex formed by the 10-23 DNA enzyme bound to an RNA substrate was determined by X-ray diffraction at 3.0 A resolution. The 82-nucleotide complex contains two strands of DNA and two strands of RNA that form five double-helical domains. The spatial arrangement of these helices is maintained by two four-way junctions that exhibit extensive base-stacking interactions and sharp turns of the phosphodiester backbone stabilized by metal ions coordinated to nucleotides at these junctions. Although it is unlikely that the structure corresponds to the catalytically active conformation of the enzyme, it represents a novel nucleic acid fold with implications for the Holliday junction structure.
About this Structure
1BR3 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme., Nowakowski J, Shim PJ, Prasad GS, Stout CD, Joyce GF, Nat Struct Biol. 1999 Feb;6(2):151-6. PMID:10048927
Page seeded by OCA on Thu Feb 21 11:58:12 2008
