1k2j
From Proteopedia
(New page: 200px<br /><applet load="1k2j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1k2j" /> '''NMR MINIMIZED AVERAGE STRUCTURE OF d(CGTACG)...) |
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| - | [[Image:1k2j.gif|left|200px]]<br /><applet load="1k2j" size=" | + | [[Image:1k2j.gif|left|200px]]<br /><applet load="1k2j" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1k2j" /> | caption="1k2j" /> | ||
'''NMR MINIMIZED AVERAGE STRUCTURE OF d(CGTACG)2'''<br /> | '''NMR MINIMIZED AVERAGE STRUCTURE OF d(CGTACG)2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Solution structures and base pair stacking of a self- complementary DNA | + | Solution structures and base pair stacking of a self- complementary DNA hexamer d(CGTACG)(2) have been studied at 5, 10 and 15 degrees C, respectively. The stacking interactions among the center base pair steps of the DNA duplex are found to improve when the terminal base pairs became less stable due to end fraying. A new structural quantity, the stacking sum (Sigma(s)), is introduced to indicate small changes in the stacking overlaps between base pairs. The improvements in the stacking overlaps to maintain the double helical conformation are probably the cause for the observed temperature dependent structural changes in double helical DNA molecule. A detailed analysis of the helical parameters, backbone torsion angles, base orientations and sugar conformations of these structures has been performed. |
==About this Structure== | ==About this Structure== | ||
| - | 1K2J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1K2J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K2J OCA]. |
==Reference== | ==Reference== | ||
Low temperature solution structures and base pair stacking of double helical d(CGTACG)(2)., Lam SL, Ip LN, J Biomol Struct Dyn. 2002 Apr;19(5):907-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11922844 11922844] | Low temperature solution structures and base pair stacking of double helical d(CGTACG)(2)., Lam SL, Ip LN, J Biomol Struct Dyn. 2002 Apr;19(5):907-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11922844 11922844] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Ip, L | + | [[Category: Ip, L N.]] |
| - | [[Category: Lam, S | + | [[Category: Lam, S L.]] |
[[Category: self-complementary dna]] | [[Category: self-complementary dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:21 2008'' |
Revision as of 11:29, 21 February 2008
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NMR MINIMIZED AVERAGE STRUCTURE OF d(CGTACG)2
Overview
Solution structures and base pair stacking of a self- complementary DNA hexamer d(CGTACG)(2) have been studied at 5, 10 and 15 degrees C, respectively. The stacking interactions among the center base pair steps of the DNA duplex are found to improve when the terminal base pairs became less stable due to end fraying. A new structural quantity, the stacking sum (Sigma(s)), is introduced to indicate small changes in the stacking overlaps between base pairs. The improvements in the stacking overlaps to maintain the double helical conformation are probably the cause for the observed temperature dependent structural changes in double helical DNA molecule. A detailed analysis of the helical parameters, backbone torsion angles, base orientations and sugar conformations of these structures has been performed.
About this Structure
1K2J is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Low temperature solution structures and base pair stacking of double helical d(CGTACG)(2)., Lam SL, Ip LN, J Biomol Struct Dyn. 2002 Apr;19(5):907-17. PMID:11922844
Page seeded by OCA on Thu Feb 21 13:29:21 2008
