1wxo
From Proteopedia
(New page: 200px<br /><applet load="1wxo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wxo, resolution 1.88Å" /> '''Structure of Archaea...) |
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| - | [[Image:1wxo.gif|left|200px]]<br /><applet load="1wxo" size=" | + | [[Image:1wxo.gif|left|200px]]<br /><applet load="1wxo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1wxo, resolution 1.88Å" /> | caption="1wxo, resolution 1.88Å" /> | ||
'''Structure of Archaeal Trans-Editing Protein AlaX in complex with zinc'''<br /> | '''Structure of Archaeal Trans-Editing Protein AlaX in complex with zinc'''<br /> | ||
==Overview== | ==Overview== | ||
| - | AlaX is the homologue of the class II alanyl-tRNA synthetase editing | + | AlaX is the homologue of the class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA(Ala). Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc. Together with mutational analysis, we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine is responsible for the discrimination of noncognate serine from cognate alanine, based on their chemical natures. Furthermore, we confirmed that the conserved Gln-584 in alanyl-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala). |
==About this Structure== | ==About this Structure== | ||
| - | 1WXO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1WXO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WXO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trans-editing]] | [[Category: trans-editing]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:49:07 2008'' |
Revision as of 13:49, 21 February 2008
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Structure of Archaeal Trans-Editing Protein AlaX in complex with zinc
Overview
AlaX is the homologue of the class II alanyl-tRNA synthetase editing domain and has been shown to exhibit autonomous editing activity against mischarged tRNA(Ala). Here, we present the structures of AlaX from the archaeon Pyrococcus horikoshii in apo form, complexed with zinc, and with noncognate amino acid l-serine and zinc. Together with mutational analysis, we demonstrated that the conserved Thr-30 hydroxyl group located near the beta-methylene of the bound serine is responsible for the discrimination of noncognate serine from cognate alanine, based on their chemical natures. Furthermore, we confirmed that the conserved Gln-584 in alanyl-tRNA synthetase, which corresponds to Thr-30 of AlaX, is also critical for discrimination. These observations strongly suggested conservation of the chemical discrimination among trans- and cis-editing of tRNA(Ala).
About this Structure
1WXO is a Single protein structure of sequence from Pyrococcus horikoshii with as ligand. Full crystallographic information is available from OCA.
Reference
Molecular basis of alanine discrimination in editing site., Sokabe M, Okada A, Yao M, Nakashima T, Tanaka I, Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11669-74. Epub 2005 Aug 8. PMID:16087889
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