1gid
From Proteopedia
(New page: 200px<br /><applet load="1gid" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gid, resolution 2.500Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1gid.jpg|left|200px]]<br /><applet load="1gid" size=" | + | [[Image:1gid.jpg|left|200px]]<br /><applet load="1gid" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gid, resolution 2.500Å" /> | caption="1gid, resolution 2.500Å" /> | ||
'''CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING'''<br /> | '''CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Group I self-splicing introns catalyze their own excision from precursor | + | Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg2+-coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome. |
==About this Structure== | ==About this Structure== | ||
| - | 1GID is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG and NCO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1GID is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GID OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a group I ribozyme domain: principles of RNA packing., Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA, Science. 1996 Sep 20;273(5282):1678-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8781224 8781224] | Crystal structure of a group I ribozyme domain: principles of RNA packing., Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA, Science. 1996 Sep 20;273(5282):1678-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8781224 8781224] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cate, J | + | [[Category: Cate, J H.]] |
| - | [[Category: Cech, T | + | [[Category: Cech, T R.]] |
| - | [[Category: Doudna, J | + | [[Category: Doudna, J A.]] |
| - | [[Category: Golden, B | + | [[Category: Golden, B L.]] |
| - | [[Category: Gooding, A | + | [[Category: Gooding, A R.]] |
| - | [[Category: Kundrot, C | + | [[Category: Kundrot, C E.]] |
[[Category: Podell, E.]] | [[Category: Podell, E.]] | ||
[[Category: Zhou, K.]] | [[Category: Zhou, K.]] | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:50:25 2008'' |
Revision as of 10:50, 21 February 2008
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CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING
Overview
Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg2+-coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome.
About this Structure
1GID is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of a group I ribozyme domain: principles of RNA packing., Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA, Science. 1996 Sep 20;273(5282):1678-85. PMID:8781224
Page seeded by OCA on Thu Feb 21 12:50:25 2008
