1koa
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(New page: 200px<br /><applet load="1koa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1koa, resolution 3.3Å" /> '''TWITCHIN KINASE FRAGM...)
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Revision as of 23:31, 24 November 2007
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TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS
Overview
The myosin-associated giant protein kinases twitchin and titin are, composed predominantly of fibronectin- and immunoglobulin-like modules. We, report the crystal structures of two autoinhibited twitchin kinase, fragments, one from Aplysia and a larger fragment from Caenorhabditis, elegans containing an additional C-terminal immunoglobulin-like domain., The structure of the longer fragment shows that the immunoglobulin domain, contacts the protein kinase domain on the opposite side from the catalytic, cleft, laterally exposing potential myosin binding residues. Together, the, structures reveal the cooperative interactions between the autoregulatory, region and the residues from the catalytic domain involved in protein, substrate binding, ATP binding, catalysis and the activation loop, and, explain the differences between the observed autoinhibitory mechanism and, the one found in the structure of calmodulin-dependent kinase I.
About this Structure
1KOA is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Giant protein kinases: domain interactions and structural basis of autoregulation., Kobe B, Heierhorst J, Feil SC, Parker MW, Benian GM, Weiss KR, Kemp BE, EMBO J. 1996 Dec 16;15(24):6810-21. PMID:9003756
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