1guc
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(New page: 200px<br /><applet load="1guc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1guc" /> '''SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM...)
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Revision as of 23:41, 24 November 2007
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SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES
Overview
The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is, unusually stable compared with other symmetric tandem GU mismatches and, occurs in the P5 helix of the group I intron of Tetrahymena thermophila., The three-dimensional solution structure of (rGAGGUCUC)2 was determined, using two-dimensional NMR and a simulated annealing protocol. The, structure is remarkably similar to the A-DNA crystal structure of, (dGGGGTCCC)2 [Kneale, G., Brown, T., & Kennard, O. (1985) J. Mol. Biol., 186, 805-814] which contains the analogous motif [sequence: see text]., Incorporation of the [sequence: see text] motif has little effect on, backbone torsion angles and helical parameters compared with standard, A-form. The only significant departure from A-form is a slight, overtwisting 5' of the G in the GU mismatch and a displacement of the, mismatches toward the minor groove. Inspection of stacking patterns of, this structure and comparison with symmetric tandem GT mismatches in A-DNA, oligonucleotides from crystal structure data suggest that electrostatics, are important in determining motif stability.
About this Structure
1GUC is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing., McDowell JA, Turner DH, Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:8916893
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