1axs
From Proteopedia
(New page: 200px<br /> <applet load="1axs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1axs, resolution 2.6Å" /> '''MATURE OXY-COPE CATA...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AXS is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with CD and HOP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AXS OCA]]. | + | 1AXS is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with CD and HOP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AXS OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: oxy-cope]] | [[Category: oxy-cope]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:37:15 2007'' |
Revision as of 06:32, 30 October 2007
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MATURE OXY-COPE CATALYTIC ANTIBODY WITH HAPTEN
Overview
Antibody catalysis provides an opportunity to examine the evolution of, binding energy and its relation to catalytic function in a system that has, many parallels with natural enzymes. Here we report such a study involving, an antibody AZ-28 that catalyses an oxy-Cope rearrangement, a pericyclic, reaction that belongs to a well studied and widely used class of reactions, in organic chemistry. Immunization with transition state analogue 1, results in a germline-encoded antibody that catalyses the rearrangement of, hexadiene 2 to aldehyde 3 with a rate approaching that of a related, pericyclic reaction catalysed by the enzyme chorismate mutase. Affinity, maturation gives antibody AZ-28, which has six amino acid substitutions, one of which results in a decrease in catalytic rate. To ... [(full description)]
About this Structure
1AXS is a [Protein complex] structure of sequences from [Homo sapiens] with CD and HOP as [ligands]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
The interplay between binding energy and catalysis in the evolution of a catalytic antibody., Ulrich HD, Mundorff E, Santarsiero BD, Driggers EM, Stevens RC, Schultz PG, Nature. 1997 Sep 18;389(6648):271-5. PMID:9305839
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