1gyh

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Revision as of 23:51, 24 November 2007

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spinqualitylabelsall models 1gyh, resolution 1.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF D158A CELLVIBRIO CELLULOSA ALPHA-L-ARABINANASE MUTANT

Overview

Cellvibrio japonicus arabinanase Arb43A hydrolyzes the alpha-1,5-linked, L-arabinofuranoside backbone of plant cell wall arabinans. The, three-dimensional structure of Arb43A, determined at 1.9 A resolution, reveals a five-bladed beta-propeller fold. Arb43A is the first enzyme, known to display this topology. A long V-shaped surface groove, partially, enclosed at one end, forms a single extended substrate-binding surface, across the face of the propeller. Three carboxylates deep in the active, site groove provide the general acid and base components for glycosidic, bond hydrolysis with inversion of anomeric configuration.

About this Structure

1GYH is a Single protein structure of sequence from Cellvibrio japonicus with CL as ligand. Active as Arabinan endo-1,5-alpha-L-arabinosidase, with EC number 3.2.1.99 Full crystallographic information is available from OCA.

Reference

Cellvibrio japonicus alpha-L-arabinanase 43A has a novel five-blade beta-propeller fold., Nurizzo D, Turkenburg JP, Charnock SJ, Roberts SM, Dodson EJ, McKie VA, Taylor EJ, Gilbert HJ, Davies GJ, Nat Struct Biol. 2002 Sep;9(9):665-8. PMID:12198486

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