This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1cx5
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1cx5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cx5" /> '''ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED...)
Next diff →
Revision as of 23:56, 24 November 2007
|
ANTISENSE DNA/RNA HYBRID CONTAINING MODIFIED BACKBONE
Overview
The solution structure of an antisense DNA.RNA hybrid duplex, d(CGCGTT-MMI-TTGCGC).r(GCGCAAAACGCG) (designated R4), containing an MMI, backbone linker [3'-CH(2)N(CH(3))-O5'], is elucidated. The structural, details of the MMI linker, its structural effects on the neighboring, residues, and the molecular basis of the MMI effects are examined. The, lipophilic N-methyl group of MMI is peripheral to the helix, assuming a, conformation that is most stable with regard to the N-O torsion angle. The, MMI linker promotes a 3'-endo conformation for the sugar moieties at both, 3'- and 5'-adjacent positions and a backbone kink involving distant, residues along the 3'-direction. Comparison of R4 with other analogous, hybrid duplexes previously studied in this laboratory reveals a new family, of low-energy helical conformations that can be accommodated in stable, duplexes and a common feature of C3'-modified sugars for adopting a, C3'-endo pucker. The results of these studies emphasize the interplay of, several factors that govern the formation of stable hybrid duplexes and, provide a basis for the understanding of the biological role of the MMI, modifications, which are important building blocks for a family of, promising chimeric antisense oligonucleotides.
About this Structure
1CX5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure of an antisense DNA.RNA hybrid duplex containing a 3'-CH(2)N(CH(3))-O-5' or an MMI backbone linker., Yang X, Han X, Cross C, Bare S, Sanghvi Y, Gao X, Biochemistry. 1999 Sep 28;38(39):12586-96. PMID:10504227
Page seeded by OCA on Sun Nov 25 02:03:59 2007
