1ky8
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(New page: 200px<br /><applet load="1ky8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ky8, resolution 2.4Å" /> '''Crystal Structure of ...)
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Revision as of 00:02, 25 November 2007
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Crystal Structure of the Non-phosphorylating glyceraldehyde-3-phosphate Dehydrogenase
Overview
The NAD(+)-dependent non-phosphorylating glyceraldehyde-3-phosphate, dehydrogenase (GAPN) from the hyperthermophilic archaeum Thermoproteus, tenax represents an archaeal member of the diverse superfamily of aldehyde, dehydrogenases (ALDHs). GAPN catalyzes the irreversible oxidation of, d-glyceraldehyde 3-phosphate to 3-phosphoglycerate. In this study, we, present the crystal structure of GAPN in complex with its natural, inhibitor NADP(+) determined by multiple anomalous diffraction methods., The structure was refined to a resolution of 2.4 A with an R-factor of, 0.21. The overall fold of GAPN is similar to the structures of ALDHs, described previously, consisting of three domains: a nucleotide-binding, domain, a catalytic domain, and an oligomerization domain. Local, differences in the active site are responsible for substrate specificity., The inhibitor NADP(+) binds at an equivalent site to the, cosubstrate-binding site of other ALDHs and blocks the enzyme in its, inactive state, possibly preventing the transition to the active, conformation. Structural comparison between GAPN from the, hyperthermophilic T. tenax and homologs of mesophilic organisms, establishes several characteristics of thermostabilization. These include, protection against heat-induced covalent modifications by reducing and, stabilizing labile residues, a decrease in number and volume of empty, cavities, an increase in beta-strand content, and a strengthening of, subunit contacts by ionic and hydrophobic interactions.
About this Structure
1KY8 is a Single protein structure of sequence from Thermoproteus tenax with NA and NAP as ligands. Active as Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)), with EC number 1.2.1.9 Full crystallographic information is available from OCA.
Reference
The crystal structure of the allosteric non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeum Thermoproteus tenax., Pohl E, Brunner N, Wilmanns M, Hensel R, J Biol Chem. 2002 May 31;277(22):19938-45. Epub 2002 Feb 12. PMID:11842090
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