1xho

From Proteopedia

---

Revision as of 00:02, 25 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1xho, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Chorismate mutase from Clostridium thermocellum Cth-682

Overview

Recently, the demands of high-throughput macromolecular crystallography, have driven continuous improvements in phasing methods, data-collection, protocols and many other technologies. Single-wavelength anomalous, scattering (SAS) phasing with chromium X-ray radiation opens a new, possibility for phasing a protein with data collected in-house and has led, to several successful examples of de novo structure solution using only, weak anomalous scatterers such as sulfur. To further reduce, data-collection time and make SAS phasing more robust, it is natural to, combine selenomethionine-derivatized protein (SeMet protein) with Cr, Kalpha radiation to take advantage of the larger anomalous scattering, signal from selenium (f = 2.28 e(-)) compared with sulfur (f = 1.14, e(-)). As reported herein, the crystal structure of a putative chorismate, mutase from Clostridium thermocellum was determined using Se-SAS with Cr, Kalpha radiation. Each protein molecule contains eight selenomethionine, residues in 148 amino-acid residues, providing a calculated Bijvoet ratio, of about 3.5% at the Cr Kalpha wavelength. A single data set to 2.2 A, resolution with approximately ninefold redundancy was collected using an, imaging-plate detector coupled with a Cr source. Structure solution, refinement and deposition to the Protein Data Bank were performed within 9, h of the availability of the scaled diffraction data. The procedure used, here is applicable to many other proteins and promises to become a routine, pathway for in-house high-throughput crystallography.

About this Structure

1XHO is a Single protein structure of sequence from Clostridium thermocellum with UNX as ligand. Full crystallographic information is available from OCA.

Reference

Away from the edge II: in-house Se-SAS phasing with chromium radiation., Xu H, Yang C, Chen L, Kataeva IA, Tempel W, Lee D, Habel JE, Nguyen D, Pflugrath JW, Ferrara JD, Arendall WB 3rd, Richardson JS, Richardson DC, Liu ZJ, Newton MG, Rose JP, Wang BC, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):960-6. Epub 2005, Jun 24. PMID:15983419

Page seeded by OCA on Sun Nov 25 02:10:13 2007