1d24
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(New page: 200px<br /><applet load="1d24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d24, resolution 1.900Å" /> '''CRYSTAL AND MOLECUL...)
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Revision as of 00:10, 25 November 2007
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CRYSTAL AND MOLECULAR STRUCTURE OF A DNA DUPLEX CONTAINING THE CARCINOGENIC LESION O6-METHYLGUANINE
Overview
The crystal and molecular structure of the first DNA duplex containing the, carcinogenic lesion O6MeG has been determined to a resolution of 1.9 A and, refined to an R factor of 19%. (d[CGC-(O6Me)GCG])2 crystallizes in the, left-handed Z DNA form and has crystal parameters and conformational, features similar to those of the parent sequence [d(CG)3]2. The methyl, groups on O6 of G4 and G10 have C5-C6-O6-O6Me torsion angles of 73 degrees, and 56 degrees, respectively, and protrude onto the major groove surface., The base-pairing conformation for the methylated G.C base pairs is of the, Watson-Crick type as opposed to a wobble-type conformation that had been, proposed in a B DNA fragment. As in other Z DNA structures, a spine of, hydration is seen in the minor groove.
About this Structure
1D24 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal and molecular structure of a DNA duplex containing the carcinogenic lesion O6-methylguanine., Ginell SL, Kuzmich S, Jones RA, Berman HM, Biochemistry. 1990 Nov 20;29(46):10461-5. PMID:2271657
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