1tjz
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(New page: 200px<br /><applet load="1tjz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tjz" /> '''Solution Structure of the Active Site Stem-L...)
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Revision as of 00:11, 25 November 2007
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Solution Structure of the Active Site Stem-Loop of the VS Ribozyme
Overview
The VS ribozyme is the largest ribozyme in its class and is also the least, structurally characterized thus far. The current working model of the VS, ribozyme locates the active site in stem-loop VI. The solution structure, of this active site loop was determined using high resolution NMR, spectroscopy. The structure reveals that the ground-state conformation of, the active site differs significantly from that determined previously from, chemical structure probing and mutational analysis of the ribozyme in its, active conformation, which contains several looped out bases. In contrast, the base-pairing scheme found for the isolated loop contains three, mismatched base-pairs: an A+-C, a G-U wobble, and a sheared G-A base-pair, and no looped out bases. Dynamics observed within the active site loop, provide insight into the mechanism by which the RNA can rearrange its, secondary structure into an "activated" conformation prior to cleavage., These findings lend support to the idea that RNA secondary structure is, more fluid than once believed and that a better understanding of structure, and dynamic features of ribozymes is required to unravel the intricacies, of their catalytic abilities.
About this Structure
1TJZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The solution structure of the VS ribozyme active site loop reveals a dynamic "hot-spot"., Flinders J, Dieckmann T, J Mol Biol. 2004 Aug 20;341(4):935-49. PMID:15328609
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