1l3z

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Revision as of 00:20, 25 November 2007

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spinqualitylabelsall models 1l3z, resolution 2.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure Analysis of an RNA Heptamer

Overview

The crystal structure of an alternating RNA heptamer r(GUAUACA) has been, determined to 2.0 A resolution and refined to an R(work) of 17.1% and, R(free) of 18.5% using 2797 reflections. The heptamer crystallized in the, space group C222 with a unit cell of a = 25.74, b = 106.58, c = 30.26 A, and two independent strands in the asymmetric unit. Each heptamer forms a, duplex with its symmetry-related strand and each duplex contains six, Watson-Crick base pairs and 3'-end adenosine overhangs. Therefore, two, kinds of duplex (duplex 1 and duplex 2) are formed. Duplexes 1 stack on, each other forming a pseudo-continuous column, which is typical of the RNA, packing mode, while duplex 2 is typical of A-DNA packing with its termini, in abutting interactions. Overhang adenine residues stack within the, duplexes with C3'-endo sugar pucker and C2'-endo sugar pucker in duplexes, 1 and 2, respectively. A Na+ ion in the crystal lattice is water bridged, to two N1 atoms of symmetry-related A7 bases.

About this Structure

1L3Z is a Protein complex structure of sequences from [1] with NA as ligand. Full crystallographic information is available from OCA.

Reference

The crystal structure of an alternating RNA heptamer r(GUAUACA) forming a six base-paired duplex with 3'-end adenine overhangs., Shi K, Pan B, Sundaralingam M, Nucleic Acids Res. 2003 Mar 1;31(5):1392-7. PMID:12595546

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