1d53
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(New page: 200px<br /><applet load="1d53" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d53, resolution 1.500Å" /> '''CRYSTAL STRUCTURE A...)
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Revision as of 00:22, 25 November 2007
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CRYSTAL STRUCTURE AT 1.5 ANGSTROMS RESOLUTION OF D(CGCICICG), AN OCTANUCLEOTIDE CONTAINING INOSINE, AND ITS COMPARISON WITH D(CGCG) AND D(CGCGCG) STRUCTURES
Overview
The octadeoxyribonucleotide d(CGCICICG) has been crystallized in space, group P(6)5(22) with unit cell dimensions of a = b = 31.0 A and c = 43.7, A, and X-ray diffraction data have been collected to 1.5-A resolution., Precession photographs and the self-Patterson function indicate that 12, base pairs of Z-conformation DNA stack along the c-axis, and the double, helices pack in a hexagonal array similar to that seen in other crystals, of Z-DNA. The structure has been solved by both Patterson deconvolution, and molecular replacement methods and refined in space group P(6)5 to an R, factor of 0.225 using 2503 unique reflections greater than 3.0 sigma (F)., Comparison of the molecules within the hexagonal lattice with highly, refined crystal structures of other Z-DNA reveals only minor, conformational differences, most notably in the pucker of the deoxyribose, of the purine residues. The DNA has multiple occupancy of C:I and C:G base, pairs, and C:I base pairs adopt a conformation similar to that of C:G base, pairs.
About this Structure
1D53 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure at 1.5-A resolution of d(CGCICICG), an octanucleotide containing inosine, and its comparison with d(CGCG) and d(CGCGCG) structures., Kumar VD, Harrison RW, Andrews LC, Weber IT, Biochemistry. 1992 Feb 11;31(5):1541-50. PMID:1737011
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