1d54
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(New page: 200px<br /><applet load="1d54" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d54, resolution 1.400Å" /> '''ANTHRACYCLINE BINDI...)
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Revision as of 00:23, 25 November 2007
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ANTHRACYCLINE BINDING TO DNA: HIGH RESOLUTION STRUCTURE OF D(TGTACA) COMPLEXED WITH 4'-EPIADRIAMYCIN
Overview
Crystallographic methods have been applied to determine the, high-resolution structure of the complex formed between the, self-complementary oligonucleotide d(TGTACA) and the anthracycline, antibiotic 4'-epiadriamycin. The complex crystallises in the tetragonal, system, space group P4(1)2(1)2 with a = 2.802 nm and c = 5.293 nm, and an, asymmetric unit consisting of a single DNA strand, one drug molecule and, 34 solvent molecules. The refinement converged with an R factor of 0.17, for the 2381 reflections with F greater than or equal to 3 sigma F in the, resolution range 0.70-0.14 nm. Two asymmetric units associate such that a, distorted B-DNA-type hexanucleotide duplex is formed incorporating two, drug molecules that are intercalated at the TpG steps. The amino sugar of, 4'-epiadriamycin binds in the minor groove of the duplex and displays, different interactions from those observed in previously determined, structures. Interactions between the hydrophilic groups of the amino sugar, and the oligonucleotide are all mediated by solvent molecules. Ultraviolet, melting measurements and comparison with other anthracycline-DNA complexes, suggest that these indirect interactions have a powerful stabilising, effect on the complex.
About this Structure
1D54 is a Protein complex structure of sequences from [1] with DM6 as ligand. Full crystallographic information is available from OCA.
Reference
Anthracycline binding to DNA. High-resolution structure of d(TGTACA) complexed with 4'-epiadriamycin., Leonard GA, Brown T, Hunter WN, Eur J Biochem. 1992 Feb 15;204(1):69-74. PMID:1740157
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