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1l8f
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(New page: 200px<br /><applet load="1l8f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1l8f, resolution 1.8Å" /> '''Structure of 20K-endo...)
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Revision as of 00:30, 25 November 2007
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Structure of 20K-endoglucanase from Melanocarpus albomyces at 1.8 A
Overview
The crystal structure of the 20K endoglucanase from the thermophilic, fungus Melanocarpus albomyces (Ma20k) has been determined. The structure, was refined to 1.8 A resolution using data obtained at 120 K. Ma20k, belongs to glycoside hydrolase family 45. The three-dimensional structures, of endoglucanase V (EGV) from the fungus Humicola insolens and of an, endoglucanase from H. grisea var. thermoidea have previously been, determined. The overall structure of Ma20k consists of a six-stranded, beta-barrel domain similar to that found previously in family 45, endoglucanases. The flexible loop between strands V and VI, which was, disordered in the uncomplexed structures of the Humicola endoglucanases, but was ordered in complexed structures of EGV, is found to be well, ordered in the native structure of Ma20k. The structure of Ma20k allows, comparison between thermophilic and mesophilic proteins of family 45 and, different principles for thermostability are discussed.
About this Structure
1L8F is a Single protein structure of sequence from Melanocarpus albomyces. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.
Reference
Structure of 20K endoglucanase from Melanocarpus albomyces at 1.8 A resolution., Valjakka J, Rouvinen J, Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):765-8. Epub 2003, Mar 25. PMID:12657806
Page seeded by OCA on Sun Nov 25 02:38:13 2007
