1d91
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1d91" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d91, resolution 2.100Å" /> '''G.T BASE PAIRS IN A...)
Next diff →
Revision as of 00:39, 25 November 2007
|
G.T BASE PAIRS IN A DNA HELIX. THE CRYSTAL STRUCTURE OF D(G-G-G-G-T-C-C-C)
Overview
The synthetic deoxyoctanucleotide d(G-G-G-G-T-C-C-C) crystallizes as an, A-type DNA double helix containing two adjacent G . T base-pair, mismatches. The structure has been refined to an R-factor of 14% at 2.1 A, resolution with 104 solvent molecules located. The two G . T mismatches, adopt the "wobble" form of base-pairing. The mismatched bases are linked, by a network of water molecules interacting with the exposed functional, groups in both the major and minor grooves. The presence of two mispaired, bases in the octamer has surprisingly little effect on the global, structure of the helix or the backbone and glycosidic torsional angles., Base stacking around the mismatch is perturbed, but the central G-T step, shows particularly good base overlap, which may contribute to the, relatively high stability of this oligomer.
About this Structure
1D91 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
G . T base-pairs in a DNA helix: the crystal structure of d(G-G-G-G-T-C-C-C)., Kneale G, Brown T, Kennard O, Rabinovich D, J Mol Biol. 1985 Dec 20;186(4):805-14. PMID:4093986
Page seeded by OCA on Sun Nov 25 02:46:59 2007
