1lc6
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(New page: 200px<br /><applet load="1lc6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lc6" /> '''Solution Structure of the U6 Intramolecular ...)
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Revision as of 00:40, 25 November 2007
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Solution Structure of the U6 Intramolecular Stem-loop RNA
Overview
U6 RNA is a key component of the catalytic core of the spliceosome. A, metal ion essential for the first catalytic step of pre-mRNA splicing, binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular, stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL., The ISL binds metal ion at the U80 site with the same stereo specificity, as the intact spliceosome. The metal-binding site is adjacent to a readily, protonated C.A wobble pair. Protonation of the C.A pair and metal binding, are mutually antagonistic. These results support a ribozyme model for U6, RNA function and suggest a possible mechanism for the regulation of RNA, splicing.
About this Structure
1LC6 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure., Huppler A, Nikstad LJ, Allmann AM, Brow DA, Butcher SE, Nat Struct Biol. 2002 Jun;9(6):431-5. PMID:11992125
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