This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1tvz
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1tvz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tvz, resolution 2.00Å" /> '''Crystal structure of...)
Next diff →
Revision as of 00:43, 25 November 2007
|
Crystal structure of 3-hydroxy-3-methylglutaryl-coenzyme A synthase from Staphylococcus aureus
Overview
3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, a member of the, family of acyl-condensing enzymes, catalyzes the first committed step in, the mevalonate pathway and is a potential target for novel antibiotics and, cholesterol-lowering agents. The Staphylococcus aureus mvaS gene product, (43.2 kDa) was overexpressed in Escherichia coli, purified to homogeneity, and shown biochemically to be an HMG-CoA synthase. The crystal structure, of the full-length enzyme was determined at 2.0-A resolution, representing, the first structure of an HMG-CoA synthase from any organism. HMG-CoA, synthase forms a homodimer. The monomer, however, contains an important, core structure of two similar alpha/beta motifs, a fold that is completely, conserved among acyl-condensing enzymes. This common fold provides a, scaffold for a catalytic triad made up of Cys, His, and Asn required by, these enzymes. In addition, a crystal structure of HMG-CoA synthase with, acetoacetyl-CoA was determined at 2.5-A resolution. Together, these, structures provide the structural basis for an understanding of the, mechanism of HMG-CoA synthase.
About this Structure
1TVZ is a Single protein structure of sequence from Staphylococcus aureus with SO4 as ligand. Active as Hydroxymethylglutaryl-CoA synthase, with EC number 2.3.3.10 Full crystallographic information is available from OCA.
Reference
Staphylococcus aureus 3-hydroxy-3-methylglutaryl-CoA synthase: crystal structure and mechanism., Campobasso N, Patel M, Wilding IE, Kallender H, Rosenberg M, Gwynn MN, J Biol Chem. 2004 Oct 22;279(43):44883-8. Epub 2004 Aug 2. PMID:15292254
Page seeded by OCA on Sun Nov 25 02:50:32 2007
