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spinqualitylabelsall models 1da1, resolution 2.250Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURAL CHARACTERISATION OF THE BROMOURACIL-GUANINE BASE PAIR MISMATCH IN A Z-DNA FRAGMENT

Overview

The deoxyoligonucleotide d(BrU-G-C-G-C-G) was crystallised at pH 8.2 and, its structure analysed by X-ray diffraction. The unit cell, of dimensions, a = 17.94, b = 30.85, c = 49.94A contains four DNA duplexes in space group, P2(1)2(1)2(1). The duplexes are in the Z conformation, with four, Watson-Crick G.C base pairs and two BrU.G base pairs. The structure was, refined to an R factor of 0.16 at a resolution of 2.2A with 64 solvent, molecules located. The BrU.G base pair mismatch is of the wobble type, with both bases in the major tautomer form and hydrogen bonds linking 0-2, of BrU with N-1 of G and N3 of BrU with 0-6 of G. There is no indication, of the presence of ionised base pairs, in spite of the high pH of, crystallisation. The results are discussed in terms of the mutagenic, properties of 5- bromouracil.

About this Structure

1DA1 is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.

Reference

Structural characterisation of the bromouracil.guanine base pair mismatch in a Z-DNA fragment., Brown T, Kneale G, Hunter WN, Kennard O, Nucleic Acids Res. 1986 Feb 25;14(4):1801-9. PMID:3951996

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