1da2
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(New page: 200px<br /><applet load="1da2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1da2, resolution 1.700Å" /> '''MOLECULAR AND CRYST...)
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Revision as of 00:44, 25 November 2007
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MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO4CG): N4-METHOXYCYTOSINE/GUANINE BASE-PAIRS IN Z-DNA
Overview
The base analogue N4-methoxycytosine (mo4C) is ambivalent in its, hydrogen-bonding potential, since it forms stable base-pairs with both, adenine and guanine in oligomer duplexes. To investigate the base-pair, geometry, the structure of d(CGCGmo4CG) has been determined by, single-crystal X-ray diffraction techniques. The d(CGCGmo4CG)2, crystallized in a left-handed double helical structure (Z-type)., Refinement using 2559 reflections between 10 and 1.7 A converged with a, final R = 0.181 (Rw = 0.130) including 68 solvent molecules. The, orthorhombic crystals are in the space group P2(1)2(1)2(1), with cell, dimensions a = 18.17 A, b = 30.36 A, c = 43.93 A. The mo4C.G base-pair is, of the wobble type, with mo4C in the imino form, and the methoxy group in, the syn configuration.
About this Structure
1DA2 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Molecular and crystal structure of d(CGCGmo4CG): N4-methoxycytosine.guanine base-pairs in Z-DNA., Van Meervelt L, Moore MH, Lin PK, Brown DM, Kennard O, J Mol Biol. 1990 Dec 5;216(3):773-81. PMID:2258939
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