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1da5
From Proteopedia
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(New page: 200px<br /><applet load="1da5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1da5" /> '''SOLUTION STRUCTURE OF A DNA DUPLEX CONTAININ...)
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Revision as of 00:45, 25 November 2007
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SOLUTION STRUCTURE OF A DNA DUPLEX CONTAINING THE CIS-PT(NH3)2[D(-GTG-)-N7-(G)-N7(G)N7(G)]ADDUCT AS DETERMINED WITH HIGH FIELD NMR AND MOLECULAR MECHANICS/DYNAMICS
Overview
The solution structure of the cis-Pt-GTG adduct in the double-stranded, oligomer d(CTCTAGTGCTCAC).td(GTGAGCACTAGAG) was studied with, high-resolution NMR techniques. For model building, the distance, information obtained from two-dimensional NOE experiments was used in, molecular mechanic computations and molecular dynamic structure, refinements. The structural distortion upon platination appears to be, restricted to the base pairs Pt-G6.C21 and T7.A20; Pt-G8.C19 forms a, normal Watson-Crick base pair. T7 is positioned in the minor groove and, stacks with the highly propeller-twisted Pt-G6. There is no hydrogen, bonding between T7 and A20. The complementary strand is undistorted; A20, stacks with its flanking cytidines (C19 and C21) as in regular B-DNA. The, duplex is locally unwound (from base pair A5.T22 to G8.C19: 19 degrees), and is slightly kinked (20 degrees) at the platination site. The platinum, coordination distorts the DNA structure at the 5' side of the, platinated-GTG-sequence and changes the minor groove face.
About this Structure
1DA5 is a Protein complex structure of sequences from [1] with CPT as ligand. Full crystallographic information is available from OCA.
Reference
The solution structure of a DNA duplex containing the cis-Pt(NH3)2[d(-GTG-)-N7(G),N7(G)] adduct, as determined with high-field NMR and molecular mechanics/dynamics., van Garderen CJ, van Houte LP, Eur J Biochem. 1994 Nov 1;225(3):1169-79. PMID:7957208
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