1prp
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(New page: 200px<br /><applet load="1prp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1prp, resolution 2.100Å" /> '''CRYSTAL STRUCTURE O...)
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Revision as of 00:47, 25 November 2007
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CRYSTAL STRUCTURE OF D(CGCGAATTCGCG) COMPLEXED WITH PROPAMIDINE, A SHORT-CHAIN HOMOLOGUE OF THE DRUG PENTAMIDIN
Overview
The crystal structure of the complex between propamidine and the, self-complementary DNA dodecamer d(CGCGAATTCGCG) duplex has been, determined to a resolution of 2.1 A and a R-factor of 17.4%. The, propamidine binds to the DNA within the minor groove, with specific, hydrogen-bonding interactions from the amidinium groups to the bases and, sugar groups of the DNA, via a 4-base-pair binding site. A network of, water molecules lies over the mouth of the minor groove. The structure, provides a rationale for the superior DNA-binding properties of, propamidine as compared to pentamidine [cf. Edwards, K. J., Jenkins, T. C., & Neidle, S. (1992) Biochemistry 31, 7104-7109]. This is seen to result, from (i) decreased minor groove width at the binding site and (ii) reduced, mobility of the bound propamidine molecule.
About this Structure
1PRP is a Protein complex structure of sequences from [1] with TNT as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of d(CGCGAATTCGCG) complexed with propamidine, a short-chain homologue of the drug pentamidine., Nunn CM, Jenkins TC, Neidle S, Biochemistry. 1993 Dec 21;32(50):13838-43. PMID:8268158
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