1lhz
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(New page: 200px<br /><applet load="1lhz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1lhz, resolution 2.3Å" /> '''Structure of a Human ...)
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Revision as of 00:51, 25 November 2007
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Structure of a Human Bence-Jones Dimer Crystallized in U.S. Space Shuttle Mission STS-95: 293K
Overview
Crystals of a human (Sea) Bence-Jones dimer were produced in a capillary, by vapor diffusion under microgravity conditions in the 9 day US Space, Shuttle Mission STS-95. In comparison to ground-based experiments, nucleation was facile and spontaneous in space. Appearance of a very large, (8 x 1.6 x 1.0 mm) crystal in a short time period is a strong endorsement, for the use of microgravity to produce crystals sufficiently large for, neutron diffraction studies. The Sea dimer crystallized in the, orthorhombic space group P2(1)2(1)2(1), with a = 48.9 A, b = 85.2 A, and c, = 114.0 A. The crystals grown in microgravity exhibited significantly, lower mosaicities than those of ground-based crystals and the X-ray, diffraction data had a lower overall B factor. Three-dimensional, structures determined by X-ray analysis at two temperatures (100 and 293, K) were indistinguishable from those obtained from ground-based crystals., However, both the crystallographic R factor and the free R factor were, slightly lower in the models derived from crystals produced in, microgravity. The major difference between the two crystal growth systems, is a lack of convection and sedimentation in a microgravity environment., This environment resulted in the growth of much larger, higher-quality, crystals of the Sea Bence-Jones protein. Structurally, heretofore, unrecognized grooves on the external surfaces of the Sea and other, immunoglobulin-derived fragments are regular features and may offer, supplementary binding regions for super antigens and other elongated, ligands in the bloodstream and perivascular tissues.
About this Structure
1LHZ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Comparison of the three-dimensional structures of a human Bence-Jones dimer crystallized on Earth and aboard US Space Shuttle Mission STS-95., Terzyan SS, Bourne CR, Ramsland PA, Bourne PC, Edmundson AB, J Mol Recognit. 2003 Mar-Apr;16(2):83-90. PMID:12720277
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