1huf
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1huf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1huf, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF ...)
Next diff →
Revision as of 00:58, 25 November 2007
|
CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF THE TYROSINE PHOSPHATASE YOPH FROM YERSINIA PESTIS.
Overview
Yersinia pestis, the causative agent of bubonic plague, injects effector, proteins into the cytosol of mammalian cells that enable the bacterium to, evade the immune response of the infected organism by interfering with, eukaryotic signal transduction pathways. YopH is a modular effector, composed of a C-terminal protein tyrosine phosphatase (PTPase) domain and, a multifunctional N-terminal domain that not only orchestrates the, secretion and translocation of YopH into eukaryotic cells but also binds, tyrosine-phosphorylated target proteins to mediate substrate recognition., The crystal structure of the N-terminal domain of YopH (YopH(N); residues, 1-130) has been determined at 2.0 A resolution. The amino-acid sequences, that target YopH for secretion from the bacterium and translocation into, eukaryotic cells form integral parts of this compactly folded domain. The, structure of YopH(N) bears no resemblance to eukaryotic, phosphotyrosine-binding domains, nor is it reminiscent of any known fold., Residues that have been implicated in phosphotyrosine-dependent protein, binding are clustered together on one face of YopH(N), but the structure, does not suggest a mechanism for protein-phosphotyrosine recognition.
About this Structure
1HUF is a Single protein structure of sequence from Yersinia pestis. Active as Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Full crystallographic information is available from OCA.
Reference
Structure of the N-terminal domain of Yersinia pestis YopH at 2.0 A resolution., Evdokimov AG, Tropea JE, Routzahn KM, Copeland TD, Waugh DS, Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):793-9. Epub 2001, May 25. PMID:11375498
Page seeded by OCA on Sun Nov 25 03:06:05 2007
