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2bgc
From Proteopedia
(New page: 200px<br /> <applet load="2bgc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bgc, resolution 2.30Å" /> '''PRFA-G145S, A CONST...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2BGC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]] with DTU and DTT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BGC OCA]]. | + | 2BGC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]] with DTU and DTT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BGC OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: transcriptional regulator]] | [[Category: transcriptional regulator]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:45:09 2007'' |
Revision as of 06:40, 30 October 2007
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PRFA-G145S, A CONSTITUTIVE ACTIVE MUTANT OF THE TRANSCRIPTIONAL REGULATOR IN L.MONOCYTOGENES
Overview
Listeria monocytogenes, a Gram-positive, facultative intracellular human, pathogen, causes systemic infections with high mortality rate. The, majority of the known pathogenicity factors of L. monocytogenes is, regulated by a single transcription factor, PrfA. Hyperhaemolytic, laboratory strains of L. monocytogenes express the constitutively active, mutant PrfA(G145S) inducing virulence gene overexpression independent of, environmental conditions. PrfA belongs to the Crp/Fnr family of, transcription factors generally activated by a small effector, such as, cAMP or O(2). We present the crystal structures of wild-type PrfA, the, first Gram-positive member of the Crp/Fnr family, and of the, constitutively active mutant PrfA(G145S). Cap (Crp) has previously been, described exclusively in the ... [(full description)]
About this Structure
2BGC is a [Single protein] structure of sequence from [Listeria monocytogenes] with DTU and DTT as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif., Eiting M, Hageluken G, Schubert WD, Heinz DW, Mol Microbiol. 2005 Apr;56(2):433-46. PMID:15813735
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