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1hwq
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(New page: 200px<br /><applet load="1hwq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hwq" /> '''SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTR...)
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Revision as of 01:04, 25 November 2007
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SOLUTION STRUCTURE OF THE VS RIBOZYME SUBSTRATE STEM-LOOP
Overview
The VS ribozyme is a 154 nucleotide sequence found in certain natural, strains of Neurospora. The RNA can be divided into a substrate and a, catalytic domain. Here we present the solution structure of the substrate, RNA that is cleaved in a trans reaction by the catalytic domain in the, presence of Mg2+. The 30 nucleotide substrate RNA forms a compact helix, capped by a flexible loop. The cleavage site bulge contains three, non-canonical base-pairs, including an A+.C pair with a protonated, adenine. This adenine (A622) is a pH controlled conformational switch that, opens up the internal loop at higher pH. The possible significance of this, switch for substrate recognition and cleavage is discussed.
About this Structure
1HWQ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A pH controlled conformational switch in the cleavage site of the VS ribozyme substrate RNA., Flinders J, Dieckmann T, J Mol Biol. 2001 May 11;308(4):665-79. PMID:11350168
Page seeded by OCA on Sun Nov 25 03:11:34 2007
