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spinqualitylabelsall models 1y0q, resolution 3.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of an active group I ribozyme-product complex

Overview

Group I introns are catalytic RNAs capable of orchestrating two sequential, phosphotransesterification reactions that result in self-splicing. To, understand how the group I intron active site facilitates catalysis, we, have solved the structure of an active ribozyme derived from the orf142-I2, intron from phage Twort bound to a four-nucleotide product RNA at a, resolution of 3.6 A. In addition to the three conserved domains, characteristic of all group I introns, the Twort ribozyme has peripheral, insertions characteristic of phage introns. These elements form a ring, that completely envelops the active site, where a snug pocket for, guanosine is formed by a series of stacked base triples. The structure of, the active site reveals three potential binding sites for catalytic, metals, and invokes a role for the 2' hydroxyl of the guanosine substrate, in organization of the active site for catalysis.

About this Structure

1Y0Q is a Protein complex structure of sequences from Staphylococcus phage twort with SPK, MG and SO4 as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:15580277

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