1hz6
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1hz6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hz6, resolution 1.70Å" /> '''CRYSTAL STRUCTURES O...)
Next diff →
Revision as of 01:11, 25 November 2007
|
CRYSTAL STRUCTURES OF THE B1 DOMAIN OF PROTEIN L FROM PEPTOSTREPTOCOCCUS MAGNUS WITH A TYROSINE TO TRYPTOPHAN SUBSTITUTION
Overview
The three-dimensional structure of a tryptophan-containing variant of the, IgG-binding B1 domain of protein L has been solved in two crystal forms to, 1.7 and 1.8 A resolution. In one of the crystal forms, the entire, N-terminal histidine-tag region was immobilized through the coordination, of zinc ions and its structural conformation along with the zinc, coordination scheme were determined. However, the ordering of the, histidine tag by zinc does not affect the overall structure of the rest of, the protein. Structural comparisons of the tryptophan-containing variant, with an NMR-derived wild-type structure, which contains a tyrosine at, position 47, reveals a common fold, although the overall backbone, root-mean-square difference is 1.5 A. The Y47W substitution only caused, local rearrangement of several side chains, the most prominent of which is, the rotation of the Tyr34 side chain, resulting in a 6 A displacement of, its hydroxyl group. A small methyl-sized cavity bounded by beta-strands 1, 2 and 4 and the alpha-helix was found in the structures of the, Y47W-substituted protein L B1 domain. This cavity may be created as the, result of subsequent side-chain rearrangements caused by the Y47W, substitution. These high-resolution structures of the, tryptophan-containing variant provide a reference frame for the analysis, of thermodynamic and kinetic data derived from a series of mutational, studies of the protein L B1 domain.
About this Structure
1HZ6 is a Single protein structure of sequence from Finegoldia magna. Full crystallographic information is available from OCA.
Reference
Structures of the B1 domain of protein L from Peptostreptococcus magnus with a tyrosine to tryptophan substitution., O'Neill JW, Kim DE, Baker D, Zhang KY, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):480-7. PMID:11264576
Page seeded by OCA on Sun Nov 25 03:18:39 2007
