1u53
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(New page: 200px<br /><applet load="1u53" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u53, resolution 1.56Å" /> '''Novel X-Ray Structur...)
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Revision as of 01:11, 25 November 2007
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Novel X-Ray Structure of Na-ASP-2, a PR-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection
Overview
Human hookworm infection is a major cause of anemia and malnutrition of, adults and children in the developing world. As part of on-going efforts, to control hookworm infection, The Human Hookworm Vaccine Initiative has, identified candidate vaccine antigens from the infective L3 larval stages, of the parasite, including a family of pathogenesis-related (PR) proteins, known as the Ancylostoma-secreted proteins (ASPs). A novel crystal, structure of Na-ASP-2, a PR-1 protein secreted by infective larvae of the, human hookworm Necator americanus, has been solved to resolution limits of, 1.68 A and to an R-factor of 17% using the recombinant protein expressed, in and secreted by Pichia pastoris. The overall fold of Na-ASP-2 is a, three-layer alphabetaalpha sandwich flanked by an N-terminal loop and a, short, cysteine-rich C terminus. Our structure reveals a large central, cavity that is flanked by His129 and Glu106, two residues that are well, conserved in all parasitic nematode L3 ASPs. Na-ASP-2 has structural and, charge similarities to chemokines, which suggests that Na-ASP-2 may be an, extra-cellular ligand of an unknown receptor. Na-ASP-2 is a useful, homology model for NIF, a natural antagonistic ligand of CR3 receptor., From these modeling studies, possible binding modes were predicted. In, addition, this first structure of a PR-1 protein from parasitic helminths, may shed light on the molecular basis of host-parasite interactions.
About this Structure
1U53 is a Single protein structure of sequence from Necator americanus. Full crystallographic information is available from OCA.
Reference
X-ray structure of Na-ASP-2, a pathogenesis-related-1 protein from the nematode parasite, Necator americanus, and a vaccine antigen for human hookworm infection., Asojo OA, Goud G, Dhar K, Loukas A, Zhan B, Deumic V, Liu S, Borgstahl GE, Hotez PJ, J Mol Biol. 2005 Feb 25;346(3):801-14. Epub 2005 Jan 12. PMID:15713464
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