1y56

From Proteopedia

Revision as of 01:22, 25 November 2007

Crystal structure of L-proline dehydrogenase from P.horikoshii

Overview

Two novel types of dye-linked L-proline dehydrogenase complex (PDH1 and, PDH2) were found in a hyperthermophilic archaeon, Pyrococcus horikoshii, OT3. Here we report the first crystal structure of PDH1, which is a, heterooctameric complex (alphabeta)4 containing three different cofactors:, FAD, FMN, and ATP. The structure was determined by x-ray crystallography, to a resolution of 2.86 angstroms. The structure of the beta subunit, which is an L-proline dehydrogenase catalytic component containing FAD as, a cofactor, was similar to that of monomeric sarcosine oxidase. On the, other hand, the alpha subunit possessed a unique structure composed of a, classical dinucleotide fold domain with ATP, a central domain, an, N-terminal domain, and a Cys-clustered domain. Serving as a third, cofactor, FMN was located at the interface between the alpha and beta, subunits in a novel configuration. The observed structure suggests that, FAD and FMN are incorporated into an electron transfer system, with, electrons passing from the former to the latter. The function of ATP is, unknown, but it may play a regulatory role. Although the structure of the, alpha subunit differs from that of the beta subunit, except for the, presence of an analogous dinucleotide domain with a different cofactor, the structural characteristics of PDH1 suggest that each represents a, divergent enzyme that arose from a common ancestral flavoenzyme and that, they eventually formed a complex to gain a new function. The structural, characteristics described here reveal the PDH1 complex to be a unique, diflavin dehydrogenase containing a novel electron transfer system.

About this Structure

1Y56 is a Protein complex structure of sequences from Pyrococcus horikoshii ot3 with FE, CL, SO4, FAD, FMN, ATP and CXS as ligands. Active as Proline dehydrogenase, with EC number 1.5.99.8 Full crystallographic information is available from OCA.

Reference

Crystal structure of a novel FAD-, FMN-, and ATP-containing L-proline dehydrogenase complex from Pyrococcus horikoshii., Tsuge H, Kawakami R, Sakuraba H, Ago H, Miyano M, Aki K, Katunuma N, Ohshima T, J Biol Chem. 2005 Sep 2;280(35):31045-9. Epub 2005 Jul 15. PMID:16027125

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