1q7o
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(New page: 200px<br /><applet load="1q7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q7o" /> '''Determination of f-MLF-OH Peptide Structure ...)
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Revision as of 01:34, 25 November 2007
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Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy
Overview
The three-dimensional structure of the chemotactic peptide, N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR, (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and, 18 torsion angle constraints (on 10 angles), recorded from uniformly, (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was, calculated by means of simulated annealing and a newly developed protocol, that ensures that all of conformational space, consistent with the, structural constraints, is searched completely. The result is a, high-quality structure of a molecule that has thus far not been amenable, to single-crystal diffraction studies. The extensions of the SSNMR, techniques and computational methods to larger systems appear promising.
About this Structure
1Q7O is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy., Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:12149447
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