1i9j
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(New page: 200px<br /><applet load="1i9j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i9j, resolution 2.60Å" /> '''TESTOSTERONE COMPLEX...)
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Revision as of 01:41, 25 November 2007
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TESTOSTERONE COMPLEX STRUCTURE OF THE RECOMBINANT MONOCLONAL WILD TYPE ANTI-TESTOSTERONE FAB FRAGMENT
Overview
The monoclonal anti-testosterone antibody (3-C(4)F(5)) has a relatively, high affinity (3 x 10(8) m(-1)) with an overall good specificity profile., However, the earlier characterized binding properties have shown that both, the affinity and specificity of this antibody must be improved if it is, intended for use in clinical immunoassays. In this paper, the crystal, structures of the recombinant anti-testosterone (3-C(4)F(5)) Fab fragment, have been determined in the testosterone-bound and free form at, resolutions of 2.60 and 2.72 A, respectively. The high affinity binding of, the (3-C(4)F(5)) Fab is mainly determined by shape complementarity between, the protein and testosterone. Only one direct hydrogen bond is formed, between the hydroxyl group of the testosterone D-ring and the main-chain, oxygen of Gly100(J)H. The testosterone is deeply bound in a hydrophobic, pocket, and the close shape complementarity is mainly formed by the third, complementarity-determining regions (CDR) of the heavy and light chain., Comparison of the bound structure with the free structure indicates, conformational changes in the protein upon testosterone binding. The, conformational changes of the side chains of two residues Glu95H and, Tyr99H in the CDR-H3 are particularly essential for the binding., Interesting similarities in the binding of different steroids were also, observed upon comparison of the available structures of anti-steroid, antibodies.
About this Structure
1I9J is a Protein complex structure of sequences from Mus musculus with TES as ligand. Full crystallographic information is available from OCA.
Reference
Structural insights into steroid hormone binding: the crystal structure of a recombinant anti-testosterone Fab fragment in free and testosterone-bound forms., Valjakka J, Takkinenz K, Teerinen T, Soderlund H, Rouvinen J, J Biol Chem. 2002 Feb 8;277(6):4183-90. Epub 2001 Nov 13. PMID:11707437
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