1ia6

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spinqualitylabelsall models 1ia6, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF THE CELLULASE CEL9M OF C. CELLULOLYTICUM

Overview

Cellulases cleave the beta-1.4 glycosidic bond of cellulose. They have, been characterized as endo or exo and processive or nonprocessive, cellulases according to their action mode on the substrate. Different, types of these cellulases may coexist in the same glycoside hydrolase, family, which have been classified according to their sequence homology, and catalytic mechanism. The bacterium C. celluloyticum produces a set of, different cellulases who belong mostly to glycoside hydrolase families 5, and 9. As an adaptation of the organism to different macroscopic, substrates organizations and to maximize its cooperative digestion, it is, expected that cellulases of these families are active on the various, macroscopic organizations of cellulose chains. The nonprocessive cellulase, Cel9M is the shortest variant of family 9 cellulases (subgroup 9(C)) which, contains only the catalytic module to interact with the substrate. The, crystal structures of free native Cel9M and its complex with cellobiose, have been solved to 1.8 and 2.0 A resolution, respectively. Other, structurally known family 9 cellulases are the nonprocessive, endo-cellulase Cel9D from C. thermocellum and the processive, endo-cellulase Cel9A from T. fusca, from subgroups 9(B1) and 9(A), respectively, whose catalytic modules are fused to a second domain. These, enzymes differ in their activity on substrates with specific macroscopic, appearances. The comparison of the catalytic module of Cel9M with the two, other known GH family 9 structures may give clues to explain its substrate, profile and action mode.

About this Structure

1IA6 is a Single protein structure of sequence from Clostridium cellulolyticum with CA, ZN, NI and SO4 as ligands. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.

Reference

Crystal structure of the cellulase Cel9M enlightens structure/function relationships of the variable catalytic modules in glycoside hydrolases., Parsiegla G, Belaich A, Belaich JP, Haser R, Biochemistry. 2002 Sep 17;41(37):11134-42. PMID:12220178

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