1ybl

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NMR structure of the i-motif tetramer of d(AACCCC)

Overview

Using NMR methods, we have resolved the i-motif structures formed by, d(AACCCC) and by d(CCCCAA), two versions of the DNA sequence repeated in, the telomeric regions of the C-rich strand of tetrahymena chromosomes., Both oligonucleotides form fully symmetrical i-motif tetramers built by, intercalation of two hemiprotonated duplexes containing four C*C+ pairs., The structures are extremely stable. In the tetramer of d(AACCCC), the, outermost C*C+ pairs are formed by the cytidines of the 5' ends of the, cytidine tracts. A2 forms an A2*A2 (H6trans-N7) pair stacked to C3*C3+ and, cross-strand stacked to A1. At 0 degrees C, the lifetimes of the, hemiprotonated pairs range from 1 ms for the outermost pair to, approximately 1 h for the innermost pairs. The tetramer of d(CCCCAA), adopts two distinct intercalation topologies in slow conformational, exchange. One, whose outermost C*C+ pairs are built by the cytidines of, the 5' end and the other by those of the 3' end. In both topologies, the, adenosine bases are fairly well stacked to the adjacent C*C+ pairs. They, are not paired but form symmetrical pseudo-pairs with their H6cis amino, proton and N1 nitrogen pointing towards each other.

About this Structure

1YBL is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

i-motif solution structure and dynamics of the d(AACCCC) and d(CCCCAA) tetrahymena telomeric repeats., Esmaili N, Leroy JL, Nucleic Acids Res. 2005 Jan 12;33(1):213-24. Print 2005. PMID:15647504

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