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1qbp
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(New page: 200px<br /><applet load="1qbp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qbp, resolution 2.1Å" /> '''CRYSTAL STRUCTURE OF ...)
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Revision as of 01:48, 25 November 2007
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CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS
Overview
The X-ray crystal structure of a brominated RNA helix with four mismatched, base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to, 2.1 A using the methods of multiwavelength anomalous diffraction (MAD), applied to the bromine K-absorption edge. There are three molecules in the, asymmetric unit with unique crystal-packing environments, revealing true, conformational variability at high resolution for this sequence. The, structure shows that the sequence itself does not define a consistent, pattern of solvent molecules, with the exception of the mismatched base, pairs, implying that specific RNA-protein interactions would occur only, with the nucleotides. There are a number of significant tertiary, interactions, some of which are a result of the brominated base pairs and, others that are directly mediated by the RNA 2' hydroxyl groups. The, mismatched base pairs exhibit a solvent network as well as a stacking, pattern with their nearest neighbors that validate previous thermodynamic, analysis.
About this Structure
1QBP is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226
Page seeded by OCA on Sun Nov 25 03:55:33 2007
