1a6r
From Proteopedia
(New page: 200px<br /> <applet load="1a6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a6r, resolution 2.05Å" /> '''GAL6 (YEAST BLEOMYC...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1A6R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A6R OCA]]. | + | 1A6R is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]] with SO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Sites: A73 and NUL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A6R OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: self-compartmentalizing protease]] | [[Category: self-compartmentalizing protease]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:48:36 2007'' |
Revision as of 06:43, 30 October 2007
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GAL6 (YEAST BLEOMYCIN HYDROLASE) MUTANT C73A
Overview
The Gal6 protease is in a class of cysteine peptidases identified by their, ability to inactivate the anti-cancer drug bleomycin. The protein forms a, barrel structure with the active sites embedded in a channel as in the, proteasome. In Gal6 the C termini lie in the active site clefts. We show, that Gal6 acts as a carboxypeptidase on its C terminus to convert itself, to an aminopeptidase and peptide ligase. The substrate specificity of the, peptidase activity is determined by the position of the C terminus of Gal6, rather than the sequence of the substrate. We propose a model to explain, these diverse activities and Gal6's singular ability to inactivate, bleomycin.
About this Structure
1A6R is a [Single protein] structure of sequence from [Saccharomyces cerevisiae] with SO4 as [ligand]. Structure known Active Sites: A73 and NUL. Full crystallographic information is available from [OCA].
Reference
The unusual active site of Gal6/bleomycin hydrolase can act as a carboxypeptidase, aminopeptidase, and peptide ligase., Zheng W, Johnston SA, Joshua-Tor L, Cell. 1998 Apr 3;93(1):103-9. PMID:9546396
Page seeded by OCA on Tue Oct 30 08:48:36 2007
