1ma8
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(New page: 200px<br /><applet load="1ma8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ma8, resolution 1.3Å" /> '''A-DNA decamer GCGTA(U...)
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Revision as of 01:51, 25 November 2007
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A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine
Overview
Selenium was incorporated into an oligodeoxynucleotide in the form of, 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex, left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was, determined by the multiwavelength anomalous dispersion (MAD) technique and, refined to a resolution of 1.3 A, demonstrating that selenium can, selectively substitute oxygen in DNA and that the resulting compounds are, chemically stable. Since derivatization at the 2'-alpha-position with, selenium does not affect the preference of the sugar for the C3'-endo, conformation, this strategy is suitable for incorporating selenium into, RNA. The availability of selenium-containing nucleic acids for, crystallographic phasing offers an attractive alternative to the commonly, used halogenated pyrimidines.
About this Structure
1MA8 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle. Multiwavelength anomalous dispersion., Teplova M, Wilds CJ, Wawrzak Z, Tereshko V, Du Q, Carrasco N, Huang Z, Egli M, Biochimie. 2002 Sep;84(9):849-58. PMID:12458077
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