1uhx

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spinqualitylabelsall models 1uhx, resolution 2.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of d(GCGAGAGC): the base-intercalated duplex

Overview

DNA fragments containing the sequence d(GCGAAAGC) prefer to adopt a, base-intercalated (zipper-like) duplex in the crystalline state. To, investigate effects of point mutation at the 5th residue on the structure, two crystal structures of d(GCGAGAGC) and d(GCGATAGC) have been determined, by X-ray crystallography. In the respective crystals, the two octamers, related by a crystallographic two-fold symmetry are aligned in an, anti-parallel fashion and associated to each other to form a duplex, suggesting that the base-intercalated duplex is stable even when the 5th, residue is mutated with other bases. The sheared G3:A6 pair formation, makes the two phosphate backbones closer and facilitates formation of the, A-X*-X-A* base-intercalated motif. The three duplexes are assembled around, the three-fold axis, and their 3rd and 4th residues are bound to the, hexamine cobalt chloride. The central 5th residues are bound to another, cation.

About this Structure

1UHX is a Protein complex structure of sequences from [1] with MG, CL, NA and NCO as ligands. Full crystallographic information is available from OCA.

Reference

X-ray analyses of d(GCGAXAGC) containing G and T at X: the base-intercalated duplex is still stable even in point mutants at the fifth residue., Kondo J, Umeda S, Fujita K, Sunami T, Takenaka A, J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):117-20. Epub 2003 Nov 28. PMID:14646150

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