1qet
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1qet" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qet" /> '''TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTU...)
Next diff →
Revision as of 01:54, 25 November 2007
|
TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
Overview
The symmetric, tandem GU mismatch motifs, and , which only differ in the, mismatch order, have an average difference in thermodynamic stability of 2, kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing, these motifs indicate the effect is largely localized to the mismatches, and adjacent base pairs. The three-dimensional structures of two, representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by, two-dimensional NMR and a simulated annealing protocol. Local deviations, are similar to other intrahelical GU mismatches with little effect on, backbone torsion angles and a slight overtwisting between the base pair 5', of the G of the mismatch and the mismatch itself. Comparisons of the, resulting stacking patterns along with electrostatic potential maps, suggest that interactions between highly negative electrostatic regions, between base pairs may play a role in the observed thermodynamic, differences.
About this Structure
1QET is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2., McDowell JA, He L, Chen X, Turner DH, Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950
Page seeded by OCA on Sun Nov 25 04:02:15 2007
