1e5j
From Proteopedia
(New page: 200px<br /> <applet load="1e5j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e5j, resolution 1.85Å" /> '''ENDOGLUCANASE CEL5A...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1E5J is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]] with GLC, SGC and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E5J OCA]]. | + | 1E5J is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_agaradhaerens Bacillus agaradhaerens]] with GLC, SGC and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Hydrolase Hydrolase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Site: ACI. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E5J OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:49:46 2007'' |
Revision as of 06:45, 30 October 2007
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ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHAERENS IN THE TETRAGONAL CRYSTAL FORM IN COMPLEX WITH METHYL-4II-S-ALPHA-CELLOBIOSYL-4II-THIO BETA-CELLOBIOSIDE
Overview
A new class of inhibitors for beta-D-glycoside hydrolases, in which a, single alpha-(1-->4)-glycosidic bond is incorporated into an otherwise, all-beta-(1-->4)-linked oligosaccharide, is described. Such mixed, beta/alpha-linkage cellooligosaccharides are not transition-state mimics, but instead are capable of utilising binding energy from numerous, subsites, spanning either side of the catalytic centre, without the need, for substrate distortion. This binding is significant; a mixed, alpha/beta-D-tetrasaccharide acts competitively on a number of cellulases, displaying inhibition constants in the range of 40-300 microM. Using the, Bacillus agaradhaerens enzyme Cel5A as a model system, one such mixed, beta/alpha-cellooligosaccharide, methyl, ... [(full description)]
About this Structure
1E5J is a [Single protein] structure of sequence from [Bacillus agaradhaerens] with GLC, SGC and CA as [ligands]. Active as [Hydrolase], with EC number [3.2.1.4]. Structure known Active Site: ACI. Full crystallographic information is available from [OCA].
Reference
Mixed-linkage cellooligosaccharides: a new class of glycoside hydrolase inhibitors., Fort S, Varrot A, Schulein M, Cottaz S, Driguez H, Davies GJ, Chembiochem. 2001 May 4;2(5):319-25. PMID:11828460
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