1yod
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(New page: 200px<br /><applet load="1yod" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yod, resolution 1.80Å" /> '''Crystal structure of...)
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Revision as of 02:19, 25 November 2007
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Crystal structure of a water soluble analog of phospholamban
Overview
Phospholamban (PLB) is a pentameric transmembrane protein that regulates, the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes., We previously described the computational design of a water-soluble, variant of phospholamban, WSPLB, which reproduced many of the structural, and functional properties of the native membrane-soluble protein. While, the full-length WSPLB forms a pentamer in solution, a truncated variant, forms very stable tetramers. To obtain insight into the tetramer-pentamer, cytoplasmic switch, we solved the crystal structure of the truncated, construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu, residues at a- and Ile at d-positions from residues 31-52. The crystal, structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric, coiled coil. This topology contrasts with the parallel topology of an, analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat., Analysis of these structures revealed how the nature of the partially, exposed residues at e- and g-positions influence the topology formed by, the bundle. We also constructed a model for the pentameric form of PLB, using the coiled-coil parameters derived from a single monomer in the, tetrameric structure. This model suggests that both buried and interfacial, hydrogen bonds are important for stabilizing the parallel pentamer.
About this Structure
1YOD is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:15826670
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