1v47

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spinqualitylabelsall models 1v47, resolution 2.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of ATP sulfurylase from Thermus thermophillus HB8 in complex with APS

Overview

ATP sulfurylase (ATPS) is a ubiquitous enzyme that catalyzes the transfer, of the adenylyl group from ATP to inorganic sulfate, producing adenosine, 5'-phosphosulfate (APS) and pyrophosphate. The crystal structure of ATPS, from Thermus thermophilus HB8 (TtATPS, 347 amino acid residues) in complex, with APS was determined at 2.5 A resolution. TtATPS is composed of three, domains [domain I (residues 1-134), domain II (residues 135-290), and, domain III (residues 291-347)], like the Riftia pachyptila symbiont ATPS, but lacks a fourth domain present in ATPSs from the yeast Saccharomyces, cerevisiae and from the fungus Penicillium chrysogenum. TtATPS forms a, dimer in the crystal, and the manner of subunit association is different, from that observed in dimeric R. pachyptila symbiont ATPS and in the, hexameric S. cerevisiae and P. chrysogenum ATPSs. APS is located in the, active site of TtATPS, which contains several motifs (QXRN, HXXH, and GRD), conserved in ATPSs. Unexpectedly, TtATPS binds one metal ion per subunit, in domain III. XAFS measurement of the crystal and the Bijvoet difference, Fourier map unambiguously characterized the metal ion as a zinc ion. The, zinc ion is tetrahedrally coordinated by Cys294, Cys297, Cys306, and, His310, and could not be removed from the protein by treatment with EDTA., The zinc ion binding site is far from the active site. Because all four, residues coordinated to the zinc ion are conserved in the ATPSs from, thermophilic bacteria such as Archaeoglobus fulgidus, Pyrococcus abyssi, and Sulfolobus solfataricus, zinc ion chelation may contribute to the, thermal stability of these ATPSs.

About this Structure

1V47 is a Single protein structure of sequence from Thermus thermophilus with ZN, CL, NA and ADX as ligands. Active as Sulfate adenylyltransferase, with EC number 2.7.7.4 Full crystallographic information is available from OCA.

Reference

Crystal structure of a novel zinc-binding ATP sulfurylase from Thermus thermophilus HB8., Taguchi Y, Sugishima M, Fukuyama K, Biochemistry. 2004 Apr 13;43(14):4111-8. PMID:15065853

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