1zb6
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(New page: 200px<br /><applet load="1zb6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zb6, resolution 1.95Å" /> '''Co-Crystal Structure...)
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Revision as of 02:40, 25 November 2007
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Co-Crystal Structure of ORF2 an Aromatic Prenyl Transferase from Streptomyces sp. strain cl190 complexed with GSPP and 1,6-dihydroxynaphtalene
Overview
The anti-oxidant naphterpin is a natural product containing a, polyketide-based aromatic core with an attached 10-carbon geranyl group, derived from isoprenoid (terpene) metabolism. Hybrid natural products such, as naphterpin that contain 5-carbon (dimethylallyl), 10-carbon (geranyl), or 15-carbon (farnesyl) isoprenoid chains possess biological activities, distinct from their non-prenylated aromatic precursors. These hybrid, natural products represent new anti-microbial, anti-oxidant, anti-inflammatory, anti-viral and anti-cancer compounds. A small number of, aromatic prenyltransferases (PTases) responsible for prenyl group, attachment have only recently been isolated and characterized. Here we, report the gene identification, biochemical characterization and, high-resolution X-ray crystal structures of an architecturally novel, aromatic PTase, Orf2 from Streptomyces sp. strain CL190, with substrates, and substrate analogues bound. In vivo, Orf2 attaches a geranyl group to a, 1,3,6,8-tetrahydroxynaphthalene-derived polyketide during naphterpin, biosynthesis. In vitro, Orf2 catalyses carbon-carbon-based and, carbon-oxygen-based prenylation of a diverse collection of, hydroxyl-containing aromatic acceptors of synthetic, microbial and plant, origin. These crystal structures, coupled with in vitro assays, provide a, basis for understanding and potentially manipulating the regio-specific, prenylation of aromatic small molecules using this structurally unique, family of aromatic PTases.
About this Structure
1ZB6 is a Protein complex structure of sequences from Streptomyces sp. with MG, NO3, GST and DIN as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for the promiscuous biosynthetic prenylation of aromatic natural products., Kuzuyama T, Noel JP, Richard SB, Nature. 2005 Jun 16;435(7044):983-7. PMID:15959519
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