2wdl
From Proteopedia
| Line 20: | Line 20: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2WDL is a 31 chains structure | + | 2WDL is a 31 chains structure with sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDL OCA]. |
==Reference== | ==Reference== | ||
<ref group="xtra">PMID:19363482</ref><references group="xtra"/> | <ref group="xtra">PMID:19363482</ref><references group="xtra"/> | ||
| + | [[Category: 70S Ribosomes]] | ||
| + | [[Category: RCSB PDB Molecule of the Month]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Kelley, A C.]] | [[Category: Kelley, A C.]] | ||
| Line 46: | Line 48: | ||
[[Category: Zinc]] | [[Category: Zinc]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 21 09:40:08 2010'' |
Revision as of 07:40, 21 January 2010
STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A- AND P-SITE TRNAS, AND E-SITE TRNA. THIS FILE CONTAINS THE 50S SUBUNIT FOR MOLECULE I.
Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.
Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome., Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V, Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2WDL is a 31 chains structure with sequences from Thermus thermophilus. The January 2010 RCSB PDB Molecule of the Month feature on 70S Ribosomes by David Goodsell is 10.2210/rcsb_pdb/mom_2010_1. Full crystallographic information is available from OCA.
Reference
- Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V. Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome. Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482 doi:10.1038/nsmb.1577
Page seeded by OCA on Thu Jan 21 09:40:08 2010
Categories: 70S Ribosomes | RCSB PDB Molecule of the Month | Thermus thermophilus | Kelley, A C. | Loakes, D. | Ramakrishnan, V. | Voorhees, R M. | Weixlbaumer, A. | Metal-binding | Methylation | Mrna | Paromomycin | Repressor | Ribonucleoprotein | Ribosomal protein | Ribosome | Rna-binding | Rrna-binding | Translation | Translation regulation | Trna | Trna-binding | Zinc
