3k3f
From Proteopedia
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==About this Structure== | ==About this Structure== | ||
| - | 3K3F is a 1 chain structure | + | 3K3F is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._hildenborough Desulfovibrio vulgaris str. hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Urea transport]] | [[Category: Urea transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 28 14:58:46 2010'' |
Revision as of 12:58, 28 January 2010
Crystal Structure of the Urea Transporter from Desulfovibrio Vulgaris
Urea is highly concentrated in the mammalian kidney to produce the osmotic gradient necessary for water re-absorption. Free diffusion of urea across cell membranes is slow owing to its high polarity, and specialized urea transporters have evolved to achieve rapid and selective urea permeation. Here we present the 2.3 A structure of a functional urea transporter from the bacterium Desulfovibrio vulgaris. The transporter is a homotrimer, and each subunit contains a continuous membrane-spanning pore formed by the two homologous halves of the protein. The pore contains a constricted selectivity filter that can accommodate several dehydrated urea molecules in single file. Backbone and side-chain oxygen atoms provide continuous coordination of urea as it progresses through the filter, and well-placed alpha-helix dipoles provide further compensation for dehydration energy. These results establish that the urea transporter operates by a channel-like mechanism and reveal the physical and chemical basis of urea selectivity.
Crystal structure of a bacterial homologue of the kidney urea transporter., Levin EJ, Quick M, Zhou M, Nature. 2009 Dec 10;462(7274):757-61. Epub . PMID:19865084
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3K3F is a 1 chain structure with sequence from Desulfovibrio vulgaris str. hildenborough. Full crystallographic information is available from OCA.
Reference
- Levin EJ, Quick M, Zhou M. Crystal structure of a bacterial homologue of the kidney urea transporter. Nature. 2009 Dec 10;462(7274):757-61. Epub . PMID:19865084 doi:10.1038/nature08558
Page seeded by OCA on Thu Jan 28 14:58:46 2010
Categories: Desulfovibrio vulgaris str. hildenborough | Levin, E J. | NYCOMPS, New York Consortium on Membrane Protein Structure. | Zhou, M. | Channel | Membrane protein | New york consortium on membrane protein structure | Nycomp | Protein structure initiative | Psi-2 | Structural genomic | Transport protein | Transporter | Urea transport
